TY - JOUR ID - 701476 TI - Isolation of Lysozyme from Chicken Egg White by Surface Charged Membranes JO - Journal of Membrane Science and Research JA - JMSR LA - en SN - AU - Nandala, Shiva Prasad AU - Tallam, Aarti AU - Choudhury, Namita Roy AU - Sridhar, Sundergopal AU - Bhargava, Suresh K AD - RMIT University AD - CSIR-IICT AD - Membrane Separations Laboratory, Chemical Engineering DivisionCSIR-Indian Institute of Chemical TechnologyHyderabadIndia Y1 - 2023 PY - 2023 VL - 9 IS - 3 SP - EP - KW - Sulfonated polyethersulfone KW - Polyethylene glycol KW - Antifouling KW - Lysozyme separation KW - Morphology DO - 10.22079/jmsr.2023.562862.1568 N2 - The fabrication of innovative and resourceful ultrafiltration membranes for the separation of protein mixtures is significantly required, principally in the food and pharmaceutical manufacturing sectors. The current research focuses on the preparation of surface-charged membranes by polyethersulfone (PES)/ sulfonated polyethersulfone (sPES) membranes blended with different molecular weight cut-off (MWCO) polyethylene glycol (PEG) for the separation of Lysozyme from natural chicken egg white (CEW). The synthesized membranes were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), contact angle, and porosity. By examining the morphological changes and the effect of physicochemical parameters, the optimized process condition to attain maximum lysozyme separation was determined. In the semi-batch mode of operation, the sPES-PEG400 membrane exhibited maximum Lysozyme transmission and high fouling resistance. The outcomes of the present study led to an in-depth understanding of the interrelationship between the PES/sPES with PEG, which sheds light on the use of membrane technology in complex protein mixture separation. UR - https://www.msrjournal.com/article_701476.html L1 - https://www.msrjournal.com/article_701476_306f36a51de347ca1837bc9134d9e432.pdf ER -